Gas Autoclave Manufacturer,Supplier and Exporter in India

This gas-heated autoclave serves teaching laboratories that run on natural or bottled gas. The aluminium body and lid, each formed without seams, close on a rubber gasket and are pulled airtight with tightening bolts and nuts. A pressure gauge and a double safety valve on the lid allow work at 15 psi over an ordinary gas ring or burner.

Gas autoclave specifications

Gas-heated sterilising practicals

Dilution blanks for a viable-count practical. Fill McCartney bottles with distilled water, loosen the caps and stand them in an autoclave basket above the water. After a hold at 15 psi and full cooling, tighten the caps. The blanks are then ready for a serial dilution of a yeast or bacterial culture.

Running a cycle on a gas ring. Light the burner with a piezo gas lighter, bring the vessel up to 15 psi on a strong flame, then turn the flame down until the gauge holds steady. One student keeps the time while another records the gauge reading at intervals through the hold.

Lid-closing drill on a cold unit. Before the first live run, each student seats the rubber gasket, sets the lid level and tightens the bolts in opposite pairs. A partner checks that the gap between lid and body is even all round before the bolts are released again.

Frequently Asked Questions About the Gas Autoclave

How should the lid bolts be tightened?

Set the lid level on the body with the gasket seated, then run every nut down finger-tight. Tighten them a little at a time in opposite pairs, so the lid stays level and the gasket presses evenly. Counting the turns on each nut helps you close the lid the same way every run.

Should the rubber gasket be greased?

Not unless the operating instructions say so. Keep it clean and dry instead, because oils and petroleum jelly can soften some rubbers. If the lid still leaks after the gasket has been cleaned and reseated, the gasket is worn and should be replaced.

How is a steady 15 psi held on a gas burner?

Bring the vessel up on a strong flame, then turn the gas down once the gauge reaches 15 psi. If the safety valve keeps releasing steam, the flame is still too high. If the needle drifts down, raise the flame slightly, and keep it centred under the base.

Is a pressure-cooker type autoclave enough for school microbiology?

For typical school loads, yes: media bottles, racks of tubes and used plates. What matters is reaching 15 psi with the air driven out and holding it for the set time. Because it runs on natural or bottled gas, it also suits rooms with no heavy electrical supply.

Why does the lid sometimes stick after the vessel has cooled?

As the steam inside condenses, the pressure can fall below that of the room and hold the lid down. Let air back in by the method the operating instructions give, then loosen the nuts in opposite pairs. Never lever the lid off with a tool.

What regular checks do the gauge and valves need?

Before each run, check that the gauge reads zero when cold and that the double safety valve moves freely. At set intervals, have a competent person examine the vessel, gauge and valves, and keep a written record of each check. Replace a gauge that no longer returns to zero.

Sterilizers and gas-bench accessories

• Electric Autoclave
• Autoclave Stainless Steel
• Gas Lighter, Piezo Electric
• Autoclave Basket
• Autoclave Bag Disposable
• Autoclave Tape

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Last updated: 14 November 2026