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Oxidase Reagent
Oxidase Reagent (Product Code: SCI-249/041) is a laboratory reagent used to detect oxidase enzymes in bacteria during identification tests in microbiology laboratories. Students and technicians use it in the oxidase test, which separates oxidase-positive organisms such as Pseudomonas from oxidase-negative members of the Enterobacteriaceae; it comes in a 30 ml bottle, a handy size among the lab chemicals on a microbiology bench. It is supplied by Jain Scientific Equipments Pvt Ltd (ScienceLab), Ambala, India.
Specifications
| Parameter | Detail |
|---|---|
| Purpose | Detecting oxidase enzymes |
| Volume | 30 ml bottle |
| Shelf life | Shelf life depends on the chemical, its grade and storage conditions; ask us for the expiry date of the batch you are quoted. |
| Product code | SCI-249/041 |
| Supplied by | Jain Scientific Equipments Pvt Ltd (ScienceLab), Ambala, India |
What Is the Oxidase Reagent Used For?
The oxidase test asks one quick question of a bacterial colony: does it produce cytochrome c oxidase? A little of the colony is rubbed on to filter paper moistened with the reagent, or the reagent is dropped on to colonies, and a colour change signals an oxidase-positive organism. It is one of the first tests used to sort Gram-negative rods, and it also features in water testing microbiology, where presumptive coliforms are expected to be oxidase-negative.
Pick colonies with a platinum loop, a plastic loop or a wooden stick, because nichrome or iron wire can give a false-positive reaction. Test fresh growth, read the result within the time stated in the instructions supplied with the reagent, and run known positive and negative cultures alongside. Many oxidase reagents darken on exposure to air and light, so keep the bottle tightly closed and cool between sessions.
Supplier
ScienceLab is the trading name of Jain Scientific Equipments Private Limited, Ambala, Haryana, which supplies the Oxidase Reagent to microbiology teaching labs, testing laboratories and dealers in India and international markets. Labs usually order the reagent with the control cultures and consumables for the same identification practical. The ScienceLab profile page has further company information.
Related Laboratory Equipment
Items that complete an oxidase test set-up:
- Enterobacter Aerogenes — an oxidase-negative culture for the negative comparison.
- Proteus Mirabilis — another oxidase-negative Gram-negative rod for practice.
- Filter Paper — moistened with reagent for the paper method.
- Pipette, Pasteur — places drops of reagent on paper or colonies.
- Petri Dish (Complete) — plates for growing the colonies to be tested.
Find more reagents and microbiology items in Laboratory Equipment.
Frequently Asked Questions
What does Oxidase Reagent detect?
Oxidase Reagent detects oxidase enzymes, namely the cytochrome c oxidase produced by some bacteria. A colour change after a colony meets the reagent indicates an oxidase-positive organism, while no colour change indicates an oxidase-negative result.
What pack size is the Oxidase Reagent?
The Oxidase Reagent is supplied in a 30 ml bottle. As the test needs only a drop or two per colony, one bottle serves many tests; state the number of bottles you need when you ask for a quotation.
Which bacteria give a positive result with Oxidase Reagent?
With Oxidase Reagent, genera such as Pseudomonas, Neisseria and Vibrio typically test oxidase-positive, while Enterobacteriaceae such as Escherichia, Enterobacter and Klebsiella test oxidase-negative. This makes the test a quick early step in identifying Gram-negative bacteria.
Which loop should be used with Oxidase Reagent?
Use a platinum loop, a plastic loop or a wooden stick when testing colonies with Oxidase Reagent. Nichrome or other iron-containing wire can oxidise the reagent itself and give a false-positive reaction.
What is the shelf life of Oxidase Reagent?
Shelf life of Oxidase Reagent depends on the chemical, its grade and storage conditions; ask us for the expiry date of the batch you are quoted. Keep the bottle tightly closed, cool and away from light between uses.
Who supplies the Oxidase Reagent?
The Oxidase Reagent is supplied by Jain Scientific Equipments Pvt Ltd (ScienceLab), Ambala, Haryana, India, to teaching and testing laboratories and to dealers in India and international markets, alone or with control cultures and consumables.
Request a Quote
To price the Oxidase Reagent, send the number of bottles and your delivery location through the contact page, or add it to the enquiry cart with cultures and plates for the same practical.
Last updated: 24 September 2026
